C型肝炎患者自体抗细胞周期抗体研究
【外文题名】A preliminary study on anti-cell cycle related autoantibody for HCV infected patients
【作者】 詹志伟
【关键词】 细胞周期 C型肝炎 自体抗体 萤光染色 抗核酸自体抗体 西方墨点法
【外文关键词】 cell cycle related autoantibody midby cell cycle cleavage furrow ANA ELISA western blot anti dsDNA anti ssDNA antii NS5a anti core
【导师姓名】张铭一
【学位名称】硕士
【学位年度】2009
【学位授予单位】暂无
【所属分类】S855.3,S852.4,R329.2
【录入时间】2009-01-01
【全文挂接】 读秀挂接
【摘要】感染HCV後自體抗體的生成與治療過程引發免疫反應的差異。因此本研究蒐集客家與閩南兩個治療成功率不同的族群C型肝炎患者血清，並以HEp-2細胞進行免疫螢光染色觀察客家與閩南兩族群螢光反應的差異和治療前後的變化，並進一步以ELISA和西方墨點法分析。研究顯示HCV患者具有特異性的細胞週期自體抗體，並可觀察到mitotic spindle fiber、centrosome、cleavage furrow、midbody等螢光特徵，且客家、閩南兩族群顯示有不同偏好的螢光反應。利用HCV core蛋白質的ELISA分析和HCV replicon細胞的西方墨點法分析發現治療前有高效價anti-core抗體以及anti-NS5A會有較好的治療結果。以紫外線誘發細胞產生蛋白質方式，發現HCV患者可能具有針對此類蛋白質的自體抗體。這些初步性資料可提供作為未來慢性C型肝炎新的研究方向。...
【外文摘要】Some previous studies had shown that HCV-infected patients could be induced autoantibodies even engendered local or systemic autoimmune diseases. In clinically, interferon-INF-) in combination with ribavirin is a major treatment for chronic hepatitis C, but only approximately 50 percent of HCV- infected patients have better prognosis. This treatment could induce autoantibodies production. At present, there is no comprehensive investigation for autoantibodies production in diference of ethnic group after HCV infection, and different in immune response for treatment. In this study, the collected HCV-infected patients’ sera from two ethnic groups of Hakka and Min-nan were different prognosis. Using HEp-2 cells for immunofluorecence stain showed that response in treatment before and after sera from Hakka and Min-nan. Then characteristic analysis was by ELISA and western blot. The results revealed HCV-infected patients had specific cell-cycle autoantibodies. The patterns were including mitotic spindle fiber, centrosome, cleavage furrow, and midbody. Two groups showed difference outcome. ELISA analysis for HCV core protein and western blot for HCV replicon cell discovered high titer anti-core and anti-NS5A could be better prognosis. UV lignt induced cell to product proteins that HCV-infected patients could detect autoantibodies against these. The preliminary data provided new directions for HCV researches in future....